DNA Replication
Cool DNA replication youtube vids:
Simple: http://www.youtube.com/watch?v=vtvvFqI5HIE
From Class: http://www.youtube.com/watch?v=yqESR7E4b_8
Funny Song: http://www.youtube.com/watch?v=dIZpb93NYlw
Simple: http://www.youtube.com/watch?v=vtvvFqI5HIE
From Class: http://www.youtube.com/watch?v=yqESR7E4b_8
Funny Song: http://www.youtube.com/watch?v=dIZpb93NYlw
Main Topics
a. replication bubbles, helicase
b. RNA polymerase
c. DNA polymerase
d. Ligase
e. continuous and fragmented replication at each replication fork – due to antiparallel nature of DNA
b. RNA polymerase
c. DNA polymerase
d. Ligase
e. continuous and fragmented replication at each replication fork – due to antiparallel nature of DNA
Blackboard Questions
- List the enzymes involved in DNA replication and their functions.
- Helicase: Unzips bases
- RNA Polymerese: primer agent for DNA polymerese
- DNA Polymerese: Grabs onto RNA polymerese and replicates the DNA strand.
- Articulate the steps involved in replicating DNA in a replication bubble.
- DNA is unwound and separated by Helicase
- Single stranded binding proteins attach to unravelled sections to prevent re-annealing of base pairs.
- RNA primase adds RNA primers to both strands of the the DNA
- DNA polymerase adds free bases continuously to the leading strand in the direction of 5’ to 3’
- The lagging strand is build discontinuously in the opposite direction that the replication fork is moving. These are split up into short fragments called Okazaki fragments.
- DNA ploymerase takes away the RNA primers and replaces them with the appropriate base pairs.